The paper Croatian Martyrs in the Light of the Eucharistic Sacrifice aims to introduce and present the martyrs of the Church's faith in Croats from the perspective of Christ's Eucharistic sacrifice. It is conceived in three chapters, through which, using the method of systematic theology, it investigates the connection between the secret faith of the Eucharistic sacrifice and martyrdom. The biblical-theological understanding of sacrifice starts from the Old Testament sacrifices, where the paschal sacrifice at the time of the exodus from Egyptian slavery stands out as a foreshadowing of our Passover Christ, which leads to a completely different New Testament understanding of the sacrifice in which Jesus Christ is both Priest, Altair and Victim. The Eucharist is Christ Himself who gives Himself to the Church and builds it up as His Body. A shorter dogmatic-theological review of the church tradition brings out the sacrificial character of the Eucharist and the martyrdom that has a stake in it, in the faithful who in their lives realize self-sacrifice following the example of the divine teacher. This is how the mystery of the Eucharistic sacrifice and the martyr's sacrifice of confessing Jesus Christ before the persecutors are connected, by which the martyr’s become partakers of the cup. The presentation of the Croatian martyrs collected in the third part showed how the Croatian martyrs of Jesus Christ bore witness to him with their life work and showed by their example the fruits of living in Eucharistic unity with Jesus, thus continuing to offer hope and strength to Christians today who are expected to provide a credible testimony of faith.